• br The role of cell type in organ specific metastasis

  2022-04-28

  

  3.5. The role of cell type in organ specific metastasis Since the results reported above suggest that the CT26 KX2-391 binds specifically to the lung through tail vein injection, we investigated whether other tumor cells exhibited the same organ specificity. To this end, mouse B16F10 melanoma,

• br In cohorts of HR and LR patients we have

  2022-04-28

  

  In cohorts of HR and LR patients, we have previously studied tissue protein biomarkers including CSF-1 (macrophage colony stimulating factor [6]) and ErbB4 (HER4) in ovary and fallopian tubes. We found that CSF-1 and ErbB4 expression in the adnexae correlates with both HR and BRCA carrier status [

• ted in red in Figure b

  2022-04-27

  

  ted in red in Figure 1b), and depressed atrophic scar with nificant reduction of regulatory T Paxilline (Tregs) in the skin. We telangiectasia most prominent in the center of the lesion conclude that the presence of pro-tumorigenic immune cells (depicted in white in Figure 1b). During 8 year

• br DOX encapsulation and release br The percentage of DOX

  2022-04-26

  

  2.5. DOX encapsulation and release The percentage of DOX encapsulation was calculated from the amount of non-encapsulated DOX. The recovered DOX from superna-tant collected upon centrifugation of the nanoparticles (10,000 rpm, 10 min). The amount of recovered DOX was determined by UV visible sp

• Journal of Controlled Release br several

  2022-04-26

  

   Journal of Controlled Release 294 (2019) 77–90 several criteria of facile fabrication, rapid gelation time, and in situ injectability, in addition to a firm basis for safety/biocompatibility [44]. Our Chlorella AuNRs BSA-Gel appeared to meet these require-ments for practical injection use.

• Kullback Leibler divergence br min br Mij log

  2022-04-25

  

  Kullback-Leibler divergence: min Mij log Mij Mij b ! X b + is the unnormalized approximation of M, i.e., M = P 3 E. The framework is applied in a hierarchical manner to increase its ability to find mutational signatures present in few samples as well as mutational signature

• br To determine whether CAF density or CAF functional heteroge

  2022-04-25

  

  To determine whether CAF density or CAF functional heteroge-neity contributes to the aggressiveness of the tumor, we isolated CAFs from 28 NSCLC resected tumors. Using the same predefined criteria, desmoplasia was assessed in 28 NSCLC tumor samples prior to extracting and culturing primary CAF Hal

• br Taking advantage of data with years of

  2022-04-24

  

  Taking advantage of data with 23 years of follow-up, the excess hazard of patients diagnosed since 23 years or more was assumed to remain asymptotically constant at the value observed around 2010 and estimated by moving averages. Other methods can be used for extrapolating survival beyond the avai

• Rottlerin br In sporadic colorectal cancer CRC

  2022-04-22

  

  In sporadic colorectal cancer (CRC), the breakdown of the epithelial barrier subsequent to oncogenic transformation leads to the translocation of bacterial products and triggers the interleukin (IL)-23–IL-17A signaling pathway (Grivennikov et al., 2012). Increased amounts of these cytokines within

• br Din OS Dodwell D Wakefield

  2022-04-22

  

  [36] Din OS, Dodwell D, Wakefield RJ, Coleman RE. Aromatase inhibitor-induced arthralgia in early breast cancer: what do we know and how can we find out more? Breast Canc Res Treat 2010;120(3):525e38. [37] Franks AL, Slansky JE. Multiple associations between a broad spectrum of autoimmune disease

• br Correlation of markers with survival and clinico

  2022-04-18

  

  3.3.4. Correlation of markers with survival and clinico-pathological parameters In the oral cancer cohort, based on the information available for follow up in 313 patients, 133 cases were disease free, while 91 cases developed recurrence (Supplementary Table-5). It is known that in oral cancer,

• MG-132 interferes with iron cellular homeostasis and alters virulence of bovine herpesvirus 1

  2022-04-17

  

  Bovine herpesvirus 1 (BoHV-1) requires an iron-replete cell host to replicate efficiently. BoHV-1 infection provokes an increase in ferritin levels and a decrease of transferrin receptor 1 (TfR-1) expression, ultimately lowering iron pool extent. Thus, cells try to limit iron availability for virus spread. It has been demonstrated that MG-132, a proteasome inhibitor, reduces BoHV-1 release. Since ferritin, the major iron storage protein in mammalian cells, undergoes proteasome-mediated degradation, herein, the influence of MG-132 on iron metabolism during BoHV-1 infection was examined. Following infection in bovine cells (MDBK), MG-132 reduced cell death and viral yield. Western blot analysis showed a significant ferritin accumulation, likely due to the inhibition of its proteasome-mediated degradation pathway. In addition, the concomitant down-regulation of TfR-1 expression, observed during infection, was counteracted by proteasome inhibitor. This trend may be explained by enhanced acidic vesicular organelles, detected by acridine orange staining, determining a reduction of intracellular pH, that promotes new synthesis of TfR-1 degraded in a recycling pathway. In addition, MG-132 influences cellular iron distribution during BoHV-1 infection, as revealed by Perls' Prussian blue staining. However, cellular iron content, evaluated by Atomic Absorption Spectrophotometry, resulted essentially unaltered. These findings reveal that MG-132 may contribute to limit cellular iron availability for virus replication thereby enhancing cell survival.

• levels in cancer cell chromosomal DNA we

  2022-04-17

  

  levels in cancer cell chromosomal DNA, we used fluorescence mi-croscopy to stain common laboratory cell lines (293T, derived from human embryonic kidney, HAP1, derived from a male with chronic myelogenous leukemia, and HeLa obtained from a cervical cancer) with a G4 DNA-structure specific antibody,

• br Figure Suppression of NF

  2022-04-17

  

  Figure 4. Suppression of NF-κB by NF-κB siRNA enhanced CX-induced apoptosis of A549RT-eto cells. number of spheroids was counted at day 6. The data are representative of three experiments (***P 3. Discussion In this study, we found that a purified compound, caged-xanthone (CX) from C. form

• br We used a semistructured interview

  2022-04-16

  

  We used a semistructured interview guide, con-structed by the two authors, in face-to-face interviews according to a qualitative study design. The guide gave a general structure to the interview, but the inter-viewer was free to use follow-up questions whenever needed for validation and further ex

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